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Phylogenetic tree based on maximum likelihood analyses of sequence data of the mitochondrial gene COI and of the three nuclear genes conserved ATPase domain (CAD), elongation factor 1-alpha (EF) and long-wavelength rhodopsin (Opsin). Numbers above branches: statistical support based on 1000 bootstrap replicates (values below 50 are omitted) in the analysis with 4 partitions (by gene); number below branches: support in analysis with 7 partitions (by codon position; a hyphen under a node indicates that this node was not recovered in the analysis); the topology is from the analysis with 4 partitions.


Hoplitis onosmaevae sp. nov., female (holotype). 3. Lateral view; 4. Head; 5. Clypeus and mandibles; 6. Metasoma, dorsal view; 7. Last sternum, ventral view; 8. Propodeum; 9. Inner spur of hind tibia, posterior view.


Hoplitis onosmaevae sp. nov., male. 10. Left antenna, dorsal view; 11. Left antenna, front view; 12. Sterna 3–6 (the metasoma was slightly extended to expose S6); 13. Terga 5–7, dorsal view; 14. Membraneous appendage of sternum 6, lateral view.


Hoplitis onosmaevae sp. nov., foraging habitat and behaviour (France, Saint-Dalmas-le-Selvage, 23.6.2020). 15. Foraging habitat with patch of the host plant, Onosma tricerosperma subsp. fastigiata; 16. Male resting on stone between two patrolling flights, with unfolded proboscis; 17. Male resting on stone between two patrolling flights; 18. Female on a flower of Onosma tricerosperma subsp. fastigiata; 19. Female concentrating nectar with widely open mandibles.


Hoplitis onosmaevae sp. nov., nesting habitat and behaviour (France, Saint-Dalmas-le-Selvage, 23.6.2020, for photographs). 20. Female in buzzing position on a flower of Onosma tricerosperma subsp. fastigiata; 21. Female leaving a flower of Onosma tricerosperma subsp. fastigiata; 22. Nesting habitat with dead trunks of larch; 23. Nest entrance plugged with sand and pebbles; 24. Female near the nest entrance, which is visible at the bottom left; 25. Cross-section of nest (P = pollen provisions).